Alright, let’s talk about how I got into this whole mess of trying to make a fake Rolex, specifically the GMT-Master II with the “Batman” bezel. I saw someone made it once before and I thought “Hey, I can do that too!”. So, I started gathering all the stuff I needed, like it is a cooking show.
First, I ordered the most important things – the enzyme and the master mix. I read a few things online, and it seemed like everyone was talking about this “High-Fidelity” stuff. Apparently, it’s super accurate, which is exactly what I needed for this project. One article mentioned the “EzPCR qPCR 2x Master Mix (SYBR green, Low Rox)” it is a mixture of thermostable DNA polymerase, dNTPs, SYBR green I dye, reaction buffer, sounds like what I need. Another one talked about “Q5 High-Fidelity 2X Master Mix” and “G-biosciences high-fidelity DNA Polymerase 2x master mix” for the enzyme, which sounded fancy. I just picked up whatever looked good and hoped for the best. When they arrived, I laid everything out on my table, feeling like a real scientist.
Mixing and Matching
Then came the hard part – actually mixing everything together. The instructions were all over the place. Some said to use this much, others said to use that much. I basically just winged it, added a bit of this, a bit of that, and stirred it all up. I even used my kitchen mixer for a bit, just to make sure everything was blended well. Don’t judge, I cleaned it after.
The Waiting Game
After that, it was just a waiting game. I put the mixture into this little machine that heats it up and cools it down repeatedly -PCR machine. Supposedly, this helps the enzyme do its thing. I left it there for what felt like forever, checking on it every few minutes like a nervous parent. You know, peeked inside, tapped the machine a few times, the usual.
Fingers Crossed
Finally, the machine beeped, signaling the end of the process. I took out the mixture, crossed my fingers, and ran a test to see if it worked. And guess what? It actually worked! I couldn’t believe it. I actually managed to do something that looked like a “High imitationRolex GMT-Master II Batman BezelQuote”, kind of.
So yeah, that’s my story. It was a wild ride, full of ups and downs, but in the end, I did it. Or at least, I think I did. I mean, it’s not perfect, but hey, it’s something, right? And the most important thing is that I learned a lot. Or not. Who knows.
- Ordered the enzyme and master mix.
- Mixed everything together, following instructions loosely.
- Ran the mixture through a PCR machine.
- Tested the final product and it worked!